Vectors and methods for assaying the regulation of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/70 (2006.01) C12N 15/85 (2006.01) C12Q 1/02 (2006.01) C12Q 1/60 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2070058

X-8131 Abstract The present invention is a method of screening for biological or chemical substances which regulate the synthesis of apolipoprotein AI, the major protein component of high density lipoprotein. Increasing the level of apolipoprotein AI thereby raises the levels of serum high density lipoprotein. A host cell is transformed with a recombinant DNA vector which comprises the regulatory region of the apolipoprotein AI gene operably linked to a reporter gene. The transformed host cell is then exposed to various substances and analyzed for the expression of the reporter gene. The invention further comprises recombinant DNA vectors and host cells.

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