Method and kit for detecting mycobacterium avium subsp....

Details Method and kit for detecting mycobacterium avium subsp.... Method and kit for detecting mycobacterium avium subsp....

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2592859

The invention relates to a method for detecting mycobacterium avium subsp. paratuberculosis (MAP) in samples of faeces, body tissues, or milk. According to said method, a DNA extract is obtained from the samples, and the DNA extract obtained is treated by means of PCR in the presence of MAP-specific primers (MAP-Primer), and tested to find out whether MAP-specific gene sequences have been amplified during the PCR. The invention also relates to a kit for carrying out said method.

L'invention concerne un procédé pour détecter Mycobacterium avium subsp. paratuberculosis (MAP) dans des échantillons d'excréments, de tissus corporels, ou de lait. Ce procédé consiste : à isoler un extrait d'ADN dans les échantillons ; à traiter l'extrait d'ADN obtenu en présence d'amorces spécifiques à MAP (amorces MAP), au moyen de la réaction en chaîne de la polymérase (PCR), et ; à vérifier si les séquences génétiques spécifiques à MAP ont été amplifiées pendant la PCR. La présente invention se rapporte en outre à un nécessaire servant à la mise en oeuvre de ce procédé.

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