Compositions and methods for generating red chemiluminescence

Details Compositions and methods for generating red chemiluminescence Compositions and methods for generating red chemiluminescence

C - Chemistry, Metallurgy

07

F

C07F 9/6558 (2006.01) C07F 9/6541 (2006.01) C12Q 1/42 (2006.01) G01N 33/533 (2006.01) G01N 33/535 (2006.01)

Patent

CA 2292124

Compounds which generate red chemiluminescence on reaction with a phosphatase enzyme are provided as well as intermediates useful for their preparation. The chemiluminescent compounds comprise a luciferin ring system and an exocyclic enol phosphate group where the position on the luciferin ring system adjacent to the double bond is disubstituted. The chemiluminescent compounds are useful alone or within compositions containing a cationic aromatic compound in methods for producing chemiluminescence. The chemiluminescent reactions can be applied in assays for phosphatase enzymes and in assays employing enzyme-labeled specific binding pairs.

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