Novel soluble cd14 antigen

Details Novel soluble cd14 antigen Novel soluble cd14 antigen

C - Chemistry, Metallurgy

07

K

C07K 14/705 (2006.01) C12P 21/02 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01)

Patent

CA 2566101

It is intended to provide a soluble CD14 antigen having the following properties 1) to 3), which is a novel protein in vivo and useful as a diagnostic maker for sepsis, and a recombinant soluble CD fragment: 1) having a molecular weight of 13.plusmn.2 kDa in SDS-PAGE under nonreducing conditions; 2) having the amino acid sequence of SEQ ID NO:1 as the N-terminal sequence; and 3) specifically binding to an antibody constructed by using a peptide having the 16 amino acid residues represented by SEQ ID NO:2 as an antigen.

L'intention est de fournir un antigène CD14 soluble ayant les propriétés 1) à 3) suivantes, lequel est une nouvelle protéine in vivo et est utile comme marqueur de diagnostic pour la sepsie, et un fragment de CD soluble recombiné : 1) ayant un poids moléculaire de 13 .plusmn. 2 kDa mesuré par SDS-PAGE dans des conditions non réductrices ; 2) ayant la séquence d'acides aminés de la SEQ ID n· : 1 comme séquence en extrémité N ; et 3) liant spécifiquement un anticorps construit en utilisant un peptide ayant les 16 résidus d'acides aminés représentés par la SEQ ID n· : 2 en tant qu'antigène.

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