Method for reducing effect of hematocrit on measurement of...

Details Method for reducing effect of hematocrit on measurement of... Method for reducing effect of hematocrit on measurement of...

G - Physics

01

N

G01N 33/50 (2006.01) G01N 21/31 (2006.01) G01N 21/55 (2006.01) G01N 21/59 (2006.01) G01N 21/78 (2006.01) G01N 21/86 (2006.01) G01N 33/49 (2006.01) G01N 33/66 (2006.01) G01N 33/92 (2006.01) G01N 33/98 (2006.01) G01N 33/487 (2006.01)

Patent

CA 2457665

A method for determining the presence and/or amount of an analyte in a sample of whole blood comprises the step of treating the sample with a nonlytic hypertonic salt compo- sition to reduce the hematocrit by reducing the size of the red blood cells. In optical detection systems, the smaller red blood cells create greater scatter, which allows a more accurate cor- rection to be applied in a dual-wavelength detection system. In electrochemical detection systems, as well as in optical detection systems, the smaller red blood cells provide less ob- struction to the diffusion of analyte and reagents in the sample, to facilitate the reactions thereof.

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