Methods for increasing the yield of fermentable sugars from...

Details Methods for increasing the yield of fermentable sugars from... Methods for increasing the yield of fermentable sugars from...

C - Chemistry, Metallurgy

12

P

C12P 19/02 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01) C12P 7/08 (2006.01) C12P 19/00 (2006.01) C13K 1/02 (2006.01) A01H 5/00 (2006.01) C12N 9/04 (2006.01)

Patent

CA 2605643

Methods for increasing yield of fermentable sugars from plant stover are provided. The methods include using plants homozygous for two brown midrib mutations, bm1 and bm3. The methods also include using plants homozygous for a mutation in a gene that results in reduced cinnamyl alcohol dehydrogenase activity, and a mutation in a gene that results in reduced 5- hydroxyconiferaldehyde/5-hydroxyconiferyl alcohol O-methyltransferase activity. The methods also include using transgenic plants that have reduced cinnamyl alcohol dehydrogenase activity and reduced 5- hydroxyconiferaldehyde/5-hydroxyconiferyl alcohol O-methyltransferase activity in comparison with wild-type plants.

L'invention concerne des procédés pour augmenter le rendement de sucres fermentables produits par le fourrage. Ces procédés consistent à utiliser un homozygote végétal pour deux mutations de nervures brunes, bm1 et bm3. Lesdits procédés consistent également à utiliser un homozygote végétal pour une mutation dans un gène, ce qui se traduit par une activité réduite de la déshydrogénase d'alcool cinnamique, et pour une mutation dans un autre gène, ce qui se traduit par une activité réduite de la O-méthyltransférase d'alcool 5-hydroxyconiferaldéhyde/5-hydroxyconiférylique. Les procédés consistent aussi à utiliser des plantes transgéniques qui réduisent l'activité de la déshydrogénase d'alcool cinnamique et de la O-méthyltransférase d'alcool 5-hydroxyconiferaldéhyde/5-hydroxyconiférylique, comparativement à des plants de type sauvage.

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