Expression of membrane proteins using an adenylyl cyclase of...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/88 (2006.01) A61K 38/51 (2006.01) C07K 14/35 (2006.01)

Patent

CA 2447110

A method for expression of membrane proteins is provided, wherein at least a portion of a nucleotide sequence coding to the membrane protein is fused to at least a portion of a nucleotide sequence coding for an adenylyl cyclase of Mycobacterium tuberculosis or a sequence at least about 70% identical to said sequence of Mycobacterium tuberculosis. In a second step the fused nucleotide sequence is introduced into an organism and/or a living cell. Another aspect of the invention is a method for searching active agents, which interact directly with adenylyl cyclases.

L'invention concerne un procédé d'expression de protéines membranaires, selon lequel au moins une partie d'une séquence nucléotidique codant pour la protéine membranaire est fusionnée avec au moins une partie d'une séquence nucléotidique codant pour une adénylcyclase de <i>Mycobacterium tuberculosis</i> ou une séquence identique au moins à 70 % à cette séquence de <i>Mycobacterium tuberculosis</i>. Dans une seconde étape, la séquence nucléotidique fusionnée est introduite dans un organisme et/ou une cellule vivante. Dans un autre mode de réalisation, cette invention concerne un procédé permettant de chercher des agents actifs qui interagissent directement ou indirectement avec des adénylcyclases.

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