Streptomyces avermitilis gene directing the ratio of b2:b1...

Details Streptomyces avermitilis gene directing the ratio of b2:b1... Streptomyces avermitilis gene directing the ratio of b2:b1...

C - Chemistry, Metallurgy

12

N

C12N 15/60 (2006.01) C12N 1/21 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/76 (2006.01) C12P 17/18 (2006.01) C12P 19/62 (2006.01) C12Q 1/527 (2006.01)

Patent

CA 2320031

The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

Molécules polynucléotidiques comprenant des séquences de nucléotides codant un produit génique aveC, qui peuvent être utilisées pour modifier le rapport entre avermectines de classe 2 et de classe 1, ou leur quantité, produites dans des cultures de fermentation de S. avermitilis. La présente invention concerne en outre des vecteurs, des cellules hôtes et des souches mutantes de S. avermitilis dans lesquelles le gène aveC a été inactivé ou muté de manière à modifier le rapport entre avermectines de classe 2 et de classe 1, ou leur quantité.

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