Method of producing enzyme-coated surfaces and method of...

C - Chemistry – Metallurgy – 12 – N

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C12N 11/08 (2006.01) A61L 2/18 (2006.01) A61L 12/12 (2006.01) C02F 3/00 (2006.01) C12M 1/40 (2006.01)

Patent

CA 2196055

The initial action of an enzyme (for instance a catalase) on a substrate (for instance hydrogen peroxide) can be retarded by distributing a solution of the substrate across a primary and a secondary chamber and by immobilizing the enzyme on a surface in the secondary chamber. The subsequent decomposition of the substrate by the enzyme is not retarded. This method can be used in the disinfection of contact lenses by hydrogen peroxide. The surface on which the enzyme is immobilized can be formed from a nylon sheet. High levels of enzyme can be bound to a nylon sheet in which the nylon molecules have been subject to a shear during the formation of the sheet, in order to orientate the molecules within the sheet.

On peut retarder l'action initiale d'une enzyme (par exemple une catalase) sur un substrat (par exemple du peroxide d'hydrogène), en distribuant une solution de ce substrat dans une chambre primaire et une chambre secondaire, et en immobilisant ensuite l'enzyme sur une surface de cette chambre secondaire. La décomposition ultérieure du substrat par l'enzyme n'est pas retardée. Ce procédé peut être utilisé pour désinfecter des lentilles de contact au moyen de peroxide d'hydrogène. La surface sur laquelle est immobilisée l'enzyme peut être formée à partir d'une feuille de nylon. Des niveaux élevés d'enzyme peuvent être fixés à une feuille de nylon, dans laquelle les molécules de nylon ont été soumises à un cisaillement pendant la formation de la feuille, afin d'orienter les molécules à l'intérieur de ladite feuille.

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