Detection of infectious hematopoietic necrosis virus

Details Detection of infectious hematopoietic necrosis virus Detection of infectious hematopoietic necrosis virus

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2390250

A real-time PCR method for detecting and quantifying the presence or absence of Infectious Hematopoietic Necrosis Virus (IHNV) in a sample. The method includes the steps of combining with a reverse-transcribed test sample a pair of PCR primers that bind to a cDNA that has been reverse transcribed from a gene of IHNV and a labeled probe that also binds to the cDNA, and performing one or more rounds of PCR on the sample. The probe undergoes a change with each round of amplification of nucleic acid during the PCR reaction, which change causes a change in the signal provided by the label, thus permitting detection of a nucleic acid that is substantially the same as the cDNA, thus providing a detection and, if desired, a quantification, of the level of the IHNV gene in the sample.

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