Probes for detecting and identifying helicobacter pylori

Details Probes for detecting and identifying helicobacter pylori Probes for detecting and identifying helicobacter pylori

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2261576

Probes for specifically detecting and identifying H. pylori which serves as an inflammatory bacterium in digestive diseases. These probes contain a fragment formed by completely digesting a DNA carried by H. pylori with a restriction enzyme HindIII. The information of the base sequences of these probes are useful as a structural indication of primers to be used in the construction of probes specific to H. pylori by PCR or as standard sequences suitable for the comparison with genomic DNAs contained in clinical specimens.

Cette invention concerne des sondes permettant de détecter et d'identifier de manière spécifique le H. pylori, ce dernier consistant en une bactérie inflammatoire caractéristique des maladies digestives. Ces sondes contiennent un fragment qui est obtenu en effectuant la digestion complète d'un ADN porté par le H. pylori, cette digestion se faisant à l'aide d'une enzyme de restriction HindIII. Les informations relatives aux séquences de base de ces sondes peuvent être utilisées comme indications structurelles concernant les primères que l'on doit utiliser dans la construction par ACP de ces sondes spécifiques au H. pylori. Elles peuvent en outre être utilisées comme séquences standards pouvant être comparées aux ADN génomiques qui sont contenus dans des spécimens cliniques.

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