Potato .alpha. -amylase genes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/56 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/32 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2053230

DNA fragments encoding potato .alpha.-amylase, the fragments comprising a nucleotide sequence (as shown in Fig. 1-5) or a subsequence or analogue thereof, DNA fragments encoding an anti-sense mRNA molecule capable of hybridizing to an mRNA transcribed from a DNA fragment (as shown in Fig. 1-4), vectors capable of replicating in a host organism, e.g. a plant, in parti- cular a potato plant, the vectors carrying a DNA fragment as defined above, microorganisms harbouring plasmids carrying a DNA fragment as defined above. genetic constructs comprising a DNA fragment as defined above and the use of such constructs for the construction of a genetically modified plant, and genetically modified plants having an increased or reduced .alpha.-amylase activity compared to corresponding non-modified plants or an .alpha.-amylase activity which may be regulated, e.g. at low tempera- tures.

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