Means and methods for the production of adenovirus vectors

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/86 (2006.01) C12N 15/861 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2477954

The invention relates to methods and means for the production of adenoviral vectors on complementing cell lines, wherein the early region 4 open reading frame 6 (E4-orf6) encoding nucleic acid is present in the adenoviral vector and wherein the E4-orf6 gene product is compatible with one or more products of the E1 gene products provided by the complementing cell, such that the adenoviral vector can be efficiently produced by the complementing cell.

La présente invention a trait à des procédés et des moyens de production de vecteurs d'adénovirus sur des lignées cellulaires de complémentation, dans lesquels le cadre de lecture ouverte 6 de la région précoce 4 (E4-orf6) codant pour l'acide nucléique est présent dans le vecteur adénoviral et dans lesquels le produit génique de E4-orf6 est compatible avec un ou des produits des produits géniques de E1 fournis par la cellule de complémentation, de sorte que le vecteur adénoviral puisse être produit de manière efficace par la cellule de complémentation.

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