Steroid 5.alpha.-reductases

Details Steroid 5.alpha.-reductases Steroid 5.alpha.-reductases

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12Q 1/26 (2006.01) C12Q 1/32 (2006.01)

Patent

CA 2079454

Disclosed are methods and compositions for the preparation of human steroid 5.alpha.-reductases by recombinant means, as well as for the use of these enzymes in screening assays for the identification of compounds which have the ability to inhibit or otherwise alter the enzymatic function of these enzymes. In particular embodiments, methods and compositions are disclosed for the preparation of steroid 5.alpha.-reductases from human sources, as well as DNA sequences which encode these enzymes. These se- quences may be used directly in the preparation of genetic constructs, or may be employed in the preparation of hybridization probes for the selection of enzyme-encoding sequences from other sources.

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