Expression of fructose 1,6 bisphosphate aldolase in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/88 (2006.01) C12N 15/60 (2006.01)

Patent

CA 2294525

Fructose-1,6-bisphosphate aldolase (FDA) is an enzyme reversibly catalyzing the reaction converting triosephosphate into fructose-1,6-bisphosphate. In the leaf, this enzyme is located in the chloroplast (starch synthesis) and the cytosol (sucrose biosynthesis). Transgenic plants were generated that expess the E. coli fda gene in the chloroplast to improve plant yield by increasing leaf starch biosynthetic ability in particular and sucrose production in general. Leaves from plants expressing the fda transgene showed a significantly higher starch accumulation, as compared to control plants expressing the null vector, particularly early in the photoperiod, but had lower leaf sucrose. Transgenic plants also had a significantly higher root mass. Furthermore, transgenic potatoes expressing fda exhibited improved uniformity of solids.

La fructose-1,6-bisphosphate aldolase (FDA) est une enzyme destinée à catalyser de manière réversible la réaction transformant le triosephosphate en fructose-1,6-bisphosphate. Cette enzyme est située, à l'intérieur d'une feuille, dans le chloroplaste (synthèse de l'amidon), et dans le cytosol (biosynthèse du sucrose). L'invention concerne la production de plantes transgéniques exprimant le gène E. coli fda dans le chloroplaste, afin d'améliorer le rendement végétal par une augmentation de la production de sucrose en général et un accroissement de l'aptitude de la feuille à la biosynthèse de l'amidon en particulier. Tout en ayant une teneur en sucrose inférieure, les feuilles des plantes exprimant le transgène fda présentent une accumulation de l'amidon considérablement supérieure, en comparaison des plantes témoins exprimant le vecteur nul, en particulier au début de la durée d'éclairement. Ces plantes transgéniques possèdent également une masse de racines sensiblement supérieure. Les pommes de terre transgéniques exprimant le gène fda présentent en outre une uniformité de solides améliorée.

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