Recombinant flaviviruses and methods of use thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2395855

The present invention provides recombinant flaviviruses, particularly live attenuated recombinant flavivirus, which comprise exogenous nucleotide sequences which encode exogenous amino acid sequences. These recombinant flavivirus viruses comprise an exogenous nucleic acid. Infection of a host cell with a recombinant flavivirus provides for expression of the exogenous nucleic acid in a host cell and production of an antigenic polypeptide encoded by the exogenous nucleic acid. Such recombinant flavivirus are useful in eliciting an immune response to the exogenous polypeptide.

L'invention concerne des flavivirus recombinés, notamment des flavivirus recombinés vivants et atténués, lesquels comprennent des séquences nucléotidiques exogènes codant des séquences d'acides aminés exogènes. Ces flavivirus recombinés comprennent un acide nucléique exogène. L'infection d'une cellule hôte par un flavivirus recombiné provoque l'expression de l'acide nucléique exogène dans une cellule hôte, ainsi que la production d'un polypeptide antigénique codé par l'acide nucléique exogène. De tels flavivirus recombinés sont utiles pour provoquer une réponse immune dans le polypeptide exogène.

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