C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 1/12 (2006.01) C12M 3/06 (2006.01)
Patent
CA 2385362
The invention relates to a method for cultivating cells utilizing a reaction system, whereby the reaction system comprises a compartment for dialysis fluid, a compartment for cultivating cells and a membrane module connecting both compartments, whereby each membrane module has at least two compartments which are separated from each other by said membrane, one of said compartments being flooded by dialysis fluid while the other compartment is flooded by culture fluid containing cells. A first gas is introduced into said culture fluid in said compartment for cell cultivation and a second gas is introduced into said culture fluid in the membrane module. Said membrane is a functional dialysis membrane.
La présente invention concerne un procédé de culture de cellules faisant intervenir un système de réaction. Ce système de réaction comporte un récipient pour le liquide de dialyse, une chambre pour la culture de cellules, ainsi qu'un module membranaire reliant les deux chambres. Ce module membranaire présente au moins deux chambres séparées par une membrane, l'une des chambres étant traversée par un liquide de dialyse, et l'autre chambre étant traversée par un liquide de culture contenant des cellules, un premier gaz étant introduit dans le liquide de culture dans la chambre pour la culture des cellules, et un deuxième gaz étant introduit dans le liquide de culture dans le module membranaire, et la membrane étant une membrane de dialyse de manière fonctionnelle.
Eisbrenner Guenter
Mahr Klaus
Markl Herbert
Stahl Reiner
Aventis Pharma Deutschland Gmbh
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
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