Biochemical markers of ischemia

G - Physics – 01 – N

Patent

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Details

G01N 33/68 (2006.01) G01N 33/53 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2233845

The present invention provides a method for identifying the presence of spontaneous ischemia in a subject which comprises: (a) obtaining biological samples from the subject at each of a plurality of time intervals within 18 hours of the onset of a symptom; (b) measuring the amount of an angiogenic growth factor in the biological samples at each of the plurality of time intervals; and (c) comparing the amounts of the angiogenic growth factor measured in step (b), wherein decreasing amounts of the angiogenic growth factor during the time intervals is indicative of spontaneous ischemia. The present invention further provides methods for identifying the presence of ischemia induced by an exercise or a pharmaceutical composition in a subject.

Cette invention, qui porte sur un procédé permettant de constater la présence d'ischémie spontanée chez un sujet, consiste: (a) à se procurer des échantillons biologiques d'un sujet prélevés sur plusieurs intervalles de temps dans les 18 heures suivant l'apparition d'un symptôme, (b) à mesurer la quantité du facteur de croissance angiogénique dans chacun des échantillons biologiques obtenus pour chaque intervalle de temps et (c) à comparer les quantités du facteur de croissance angiogénique mesurées durant la phase (b), la dégressivité des quantités de ce facteur entre chaque intervalle de temps témoignant d'une ischémie spontanée. Cette invention porte, de surcroît, sur des procédés permettant de constater l'existence d'une ischémie déclenchée chez un sujet par un exercice ou l'absorption d'une composition pharmaceutique.

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