Method of multiplex ligase chain reaction

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07K 14/47 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01) G01N 33/558 (2006.01)

Patent

CA 2133220

2133220 9320227 PCTABS00027 The invention relates to multiplex ligase chain reaction (LCR). Two or more putative target sequences are selected. For each one, a set of four probes is used simultaneously to amplify the putative sequence if it is present in the sample. Preferably, all the amplicons are labeled with a common label/hapten and, for each different target, with a unique label/hapten. The invention also relates to an immunochromatographic strip device and method employing a diagonal array of capture spots.

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