Reverse transcription activity from bacillus...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2374494

The present invention is directed to a thermostable DNA polymerase from <i>Bacillus stearothermophilus</i> for use in reverse transcription and/or reverse transcriptase-polymerase chain reaction (RT-PCR), where said DNA polymerase shows magnesium ion dependent reverse transcriptase activity and in the substantial absence of manganese ions.

La présente invention concerne l'ADN polymérase thermostable issu de <i>Bacillus Stearothermophilus</i> et destiné à être utilisé dans une transcription inverse et/ou une transcription inverse suivie de réaction en chaîne de la polymérase (RT-PCR), dans laquelle l'ADN polymérase présente une activité de transcription inverse en présence d'ions magnésium et en l'absence substantielle d'ions manganeux.

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