Assay for protein isoforms

Details Assay for protein isoforms Assay for protein isoforms

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2515148

The present invention provides a method for assaying for a protein having at least two isoforms having different glycosylation patterns, said method comprising: contacting a sample containing said protein with a proteolytic enzyme, and detecting the content or relative content of at least one peptide fragment produced by proteolysis of said protein.

La présente invention concerne une méthode destinée au dosage d'une protéine ayant au moins deux isoformes présentant des motifs de glycosylation différents. Cette méthode consiste à mettre un échantillon contenant cette protéine en contact avec une enzyme protéolytique et à détecter la concentration ou la concentration relative d'au moins un fragment peptidique produit par protéolyse de ladite protéine.

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