Method for ice-free cryopreservation of tissue

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01) A01N 1/00 (2006.01)

Patent

CA 2753291

Method for preserving tissue including immersing the tissue in a solution having a cryoprotectant concentration of at least 75% by weight, a cooling step where the tissue is cooled to a temperature between the glass transition temperature of the solution having a cryoprotectant concentration of at least 75% by weight and - 20°C, a storage step where the tissue is stored at a temperature between the glass transition temperature of the solution and - 2O°C, a rewarming step, where the tissue is warmed, and a washing step.

La présente invention concerne un procédé qui permet de conserver un tissu, et qui comprend les étapes suivantes : l'immersion du tissu dans une solution comportant une concentration d'au moins 75 % en poids d'un agent de cryoconservation, le refroidissement pendant lequel le tissu est refroidi à une température comprise entre la température de transition vitreuse de la solution possédant une concentration d'au moins 75 % en poids d'un agent de cryoconservation et -20 °C, le stockage pendant lequel le tissu est stocké à une température comprise entre la température de transition vitreuse de la solution et -20 °C, le réchauffage, pendant lequel le tissu est réchauffé, et le lavage.

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