Improved protein production and storage in plants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) C12N 15/113 (2010.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 15/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2729375

A transgenic dicotyledonous plant having a deficiency of one or more plant seed storage proteins, further having a transgenic polynucleotide construct comprising an open reading frame operably linked to a storage protein promoter and an ER signal sequence. The polynucleotide construct encodes a protein product that can accumulate at high levels in the seed. Also pro-vided are methods of producing a heterologous protein in a plant seed.

L'invention porte sur une plante dicotylédone transgénique présentant une déficience en une ou plusieurs protéines de stockage de graines de plante et comportant en outre un produit de synthèse polynucléotidique transgénique comprenant un cadre de lecture ouvert fonctionnellement lié à un promoteur de protéine de stockage et une séquence signal ER. Le produit de synthèse polynucléotidique code pour un produit de protéine qui peut s'accumuler à des taux élevés dans la graine. L'invention porte également sur des procédés de production d'une protéine hétérologue dans une graine de plante.

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