Sensors for the detection of diols and carbohydrates using...

G - Physics – 01 – N

Patent

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G01N 33/66 (2006.01)

Patent

CA 2690304

The systems and methods disclosed herein include a sensor particle for detecting the presence of a chelatable analyte, such as glucose, wherein the sensor comprises a chromophore and a fluorescent component, such as a quantum dot. The sensor particle further comprises moieties that bind both a chelatable analyte and chromophore reversibly and competitively. In the presence of the chelatable analyte, the moieties bind the analyte, and release the chromophore. The chromophore absorbs photons of one wavelength in a free state but of a different wavelength in a bound state, and is selected to operate with the fluorescent component such that the chromophore absorbs emissions of the fluorescent substance in only one of the bound and unbound states. In certain aspects, the invention comprises methods for detecting the presence of a chelatable analyte in a medium such as water, blood plasma and urine, using the sensor particles of the invention.

L'invention concerne des systèmes et des procédés comprenant une particule détectrice pour détecter la présence d'un analyte chélatable tel que le glucose, ce détecteur contenant un chromophore et un composant fluorescent tel qu'un point quantique. La particule détectrice comprend en outre des fractions qui se lient à la fois à un analyte chélatable et à un chromophore, de manière réversible et compétitive. En présence de l'analyte chélatable, les fractions se lient à celui-ci et libèrent le chromophore. Le chromophore absorbe les photons d'une certaine longueur d'onde à l'état libre, mais ceux d'une longueur d'onde différente à l'état lié, et il est choisi de façon à coopérer avec le composant fluorescent, le chromophore n'absorbant les émissions de la substance fluorescente que dans un seul des deux états lié et libre. Certains aspects de l'invention concernent des procédés de détection de la présence d'un analyte chélatable dans un milieu tel que l'eau, le plasma sanguin et l'urine au moyen des particules détectrices décrites.

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