Plant resistance to insect pests mediated by viral proteins

Details Plant resistance to insect pests mediated by viral proteins Plant resistance to insect pests mediated by viral proteins

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A01N 37/46 (2006.01) A01N 63/02 (2006.01) C07K 14/005 (2006.01) C07K 14/08 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/33 (2006.01) C12N 15/66 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2340583

The invention involves combining a peptide toxin effective against insects, including but not limited to thrips, leaf hoppers, and beetles, with a transport peptide capable of facilitating transfer of the peptide toxin from the gut of an insect to the hemocoel. The combination can be effected by a fusion of genetic material encoding the peptide toxin and the transport peptide, such that expression of the genetic material fusion results in synthesis of a fusion protein combining the functions of both the toxin and the transport protein. Ingestion of the fusion protein by the sucking insect transfers the fusion protein into the insect's gut from which it is transferred into the hemocoel due to the functional activity of the transport peptide where the toxin exerts its toxic effect upon the insect. In a preferred embodiment, the invention is effective in control of such sucking insects as aphids, whiteflies and the like, and other vectors that transmit viruses in a circulative manner.

L'invention consiste à combiner une toxine peptidique efficace contre les insectes, parmi lesquels les thrips, les cicadelles et les coléoptères, avec un peptide de transport capable de faciliter le transfert de la toxine peptidique de l'intestin d'un insecte à son hémocèle. La combinaison peut être effectuée par la fusion de matière génétique codant pour la toxine peptidique et pour le peptide de transport, de sorte que l'expression de la fusion de matière génétique aboutit à la synthèse d'une protéine de fusion combinant les fonctions à la fois de la toxine et de la protéine de transport. L'ingestion de la protéine de fusion par des insectes suceurs a pour effet de transférer la protéine de fusion dans l'intestin de l'insecte. Sous l'effet de l'activité fonctionnelle du peptide de transport, la protéine de fusion passe dans l'hémocèle de l'insecte, où la toxine exerce son effet toxique sur l'insecte. Dans un mode de réalisation préféré, l'invention convient pour la lutte contre les insectes suceurs, telles que les pucerons, les Aleyrodidae et analogues, et contre d'autres vecteurs de transmission circulaire de virus.

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