Temperature controlled nucleic-acid detection method...

Details Temperature controlled nucleic-acid detection method... Temperature controlled nucleic-acid detection method...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12S 3/00 (2006.01) C12M 1/34 (2006.01) C12M 1/38 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2734131

The invention relates to a method that utilizes thermophilic proteases for the treatment of nucleic acids in a closed-system to be used in tandem with methods for the rapid detection of target nucleic acids present in a sample. These combined methods enable simplified, temperature-controlled devices to be used for accurate, streamline testing at the point of care for a wide variety of applications in the medical, industrial, environmental, quality control, security and research fields.

L'invention concerne un procédé qui utilise des protéases thermophiles pour le traitement d'acides nucléiques dans un système fermé, à utiliser en tandem avec des procédés pour la détection rapide d'acides nucléiques cibles présents dans un échantillon. Ces procédés combinés permettent l'utilisation de dispositifs à température contrôlée simplifiés pour des tests en ligne précis à l'endroit des soins pour une grande variété d'applications dans le domaine médical, industriel, environnemental, contrôle de qualité, sécurité et recherche.

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