Profiling reactive oxygen, nitrogen and halogen species

Details Profiling reactive oxygen, nitrogen and halogen species Profiling reactive oxygen, nitrogen and halogen species

G - Physics

01

N

G01N 33/52 (2006.01) C12M 1/34 (2006.01) G01N 33/50 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2738764

This invention provides methods, kits and systems which permit simultaneous profiling of multiple reactive oxygen species (ROS), reactive nitrogen species (RNS) and/or reactive halogen species (RHS) including reactive chlorine species (RCS) and/or reactive bromine species (RBS) through multiplexed fluorescence detection of three or more indicator probes in live cells or subcellu-lar organelles.

L'invention porte sur des procédés, des kits et des systèmes qui permettent l'établissement de profils simultané de multiples espèces oxygénées réactives (ROS), espèces azotées réactives (RNS) et/ou espèces halogénées réactives (RHS) comprenant des espèces chlorées réactives (RCS) et/ou des espèces bromées réactives (RBS) par détection de fluorescence multiplexée de trois sondes indicatrices ou plus dans des cellules vivantes ou des organelles sous-cellulaires.

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