Preservation of sensitive biological samples by vitrification

Details Preservation of sensitive biological samples by vitrification Preservation of sensitive biological samples by vitrification

C - Chemistry, Metallurgy

12

N

C12N 1/04 (2006.01) A61K 9/12 (2006.01) A61K 47/26 (2006.01) C12N 9/00 (2006.01) A61K 9/19 (2006.01)

Patent

CA 2312233

This invention discloses methods for the long-term preservation of industrial scale biological solutions and suspensions containing biologically active molecules, cells and small multicellular specimens at ambient temperatures by dehydration in amorphous very viscous liquid or glass state. The scale up method comprises the primary drying step of boiling under vacuum to form a mechanically-stable foam and a secondary drying step to increase the stability. Vitrification can subsequently be achieved by cooling the dried material to the storage temperature which is lower than the glass transition temperature.

L'invention se rapporte à des procédés de conservation à long terme et à température ambiante de grandes quantités de suspensions et solutions biologiques contenant des molécules biologiquement actives, des cellules et de petits échantillons multicellulaires. Ces procédés consistent en une déshydratation amenant ces solutions et suspensions dans un état amorphe, vitreux ou liquide très visqueux. Le procédé, conçu pour une mise en oeuvre à l'échelle commerciale, comprend une étape de séchage primaire consistant en une cuisson sous vide produisant une mousse mécaniquement stable et une étape de séchage secondaire destinée à accroître la stabilité. La vitrification peut ensuite être effectuée par refroidissement de la matière séchée à la température de stockage qui est inférieure à la température de transition vitreuse.

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