Nucleic acid integration assay

Details Nucleic acid integration assay Nucleic acid integration assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2424998

Methods for detecting integration of a polynucleotide such as a retrovirus into a target nucleic acid molecule are provided, as are methods of identifying cellular factors involved in such an integration event and of identifying agents that can modulate such integration events. Also provided are reagents and kits for performing such methods.

Cette invention se rapporte à des procédés qui servent à détecter l'intégration d'un polynucléotide, tel qu'un rétrovirus, dans une molécule d'acide nucléique cible, ainsi qu'à des procédés qui servent à identifier des facteurs cellulaires impliqués dans un tel événement d'intégration et qui servent à identifier des agents pouvant moduler de tels événements d'intégration. Cette invention concerne également des réactifs et des kits servant à réaliser ces procédés.

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