Transgenic plants expressing rabies glycoprotein g, and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) A01H 5/00 (2006.01) A61K 39/205 (2006.01) C07K 14/145 (2006.01) C07K 14/415 (2006.01) C07K 16/10 (2006.01) C07K 19/00 (2006.01) C12N 15/29 (2006.01) C12N 15/47 (2006.01) C12N 15/82 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2254115

This invention features a method for producing the glycoprotein G of the rabies virus, or a virus related to the rabies virus, characterised by: i) introducing, into a plant cell, a nucleic acid molecule comprising a chimeric coding sequence, comprising one coding sequence for the said mature viral protein G, or for an analogous protein, and a coding sequence for a N-terminal signal peptide other than the one naturally associated with the viral protein G, enabling the expression of the protein G in the cell; ii) multiplying, in the form of cell culture, transformed cells, or regeneration of whole transgenic or chimerical plants from these cells; iii) optionally extracting and purifying the glycoprotein G from the cells or plants thus obtained.

L'invention concerne un procédé pour la production de la glycoprotéine G du virus de la rage, ou d'un virus apparenté au virus de la rage, caractérisé par: i) l'introduction, dans une cellule végétale, d'une molécule d'acide nucléique comprenant une séquence codante chimérique, comportant d'une part une séquence codant pour ladite protéine G virale mature, ou pour une protéine analogue, et d'autre part, une séquence codant pour un peptide signal N- terminal autre que celui naturellement associé à la protéine G virale, l'introduction de la séquence codante chimérique permettant l'expression dans la cellule de la protéine G; ii) la multiplication, sous forme de culture cellulaire, des cellules transformées, ou la régénération de plantes entières transgéniques ou chimériques à partir de ces cellules; iii) éventuellement l'extraction et la purification de la glycoprotéine G à partir des cellules ou des tissus végétaux ainsi obtenus.

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