Multiplex oligonucleotide addition and target amplification

Details Multiplex oligonucleotide addition and target amplification Multiplex oligonucleotide addition and target amplification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2467460

Methods for appending oligonucleotides directly to nucleic acid templates, particularly to defined sites internal to single-stranded templates, are described. Appending first and second common priming sites to each of a plurality of templates of distinct sequence allows the subsequent stoichiometric amplification of a plurality of templates of distinct sequence.

Méthodes permettant d'attacher des oligonucléotides directement à des matrices d'acide nucléique, en particulier en des sites définis se trouvant à l'intérieur de matrices à simple brin. L'attache de premier et second sites d'amorçage communs à chacune des matrices parmi une pluralité de matrices à séquence distincte permet l'amplification stoechiométrique subséquente d'une pluralité de matrices à séquence distincte.

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