Method for increasing and regulating light emission from a...

Details Method for increasing and regulating light emission from a... Method for increasing and regulating light emission from a...

C - Chemistry, Metallurgy

09

K

C09K 11/07 (2006.01) C12Q 1/28 (2006.01) G01N 33/52 (2006.01) G01N 33/543 (2006.01) C12N 9/08 (2006.01)

Patent

CA 2742025

Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles., i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.

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