Regulated gene expression in yeast and methods of use

Details Regulated gene expression in yeast and methods of use Regulated gene expression in yeast and methods of use

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) C12N 15/81 (2006.01) C12Q 1/18 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2408829

The invention provides novel yeast cells comprising genes whose expression can be modulated by growth in the presence or absence of metal ions, methods for making such yeast cells, and methods of using such yeast cells for determining the requirement for expression of particular genes for the growth of viability of the yeast cells. The invention also provides methods of using such yeast cells in the isolation, screening and analysis of candidate antifungal compounds.

La présente invention concerne des cellules de levure comprenant des gènes dont l'expression peut être modulée grâce à la croissance en la présence ou en l'absence d'ions métalliques, des procédés permettant de produire lesdites levures, et des procédés d'utilisation de cellules de levure de ce type pour déterminer l'expression requise de gènes particuliers permettant la croissance ou la viabilité des cellules de levure. Cette invention concerne également des procédés d'utilisation de cellules de levure de ce type pour l'isolation, le criblage et l'analyse de composés antifongiques candidats.

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