Conditionally amplifiable bacterial artificial chromosome...

Details Conditionally amplifiable bacterial artificial chromosome... Conditionally amplifiable bacterial artificial chromosome...

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) C12N 15/64 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2308608

A system for obtaining large amounts of a genomic DNA fragment from a bacterial artificial chromosome includes a vector that has a site into which the genomic fragment can be cloned and, flanking the site are excision mediating sites. The vector also includes, between the excision mediating sites and near one of the sites, a controllable origin of replication.

L'invention concerne un système permettant d'obtenir de grandes quantités de fragment d'ADN génomique à partir d'un chromosome bactérien artificiel. Ce système comprend un vecteur doté d'un site dans lequel le clonage du fragment génomique est possible. Des sites médiateurs d'excision jouxtent le site considéré. Le vecteur comprend en outre une origine de réplication contrôlable, entre les sites médiateurs d'excision et à proximité de l'un des sites.

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