Apparatus and method for detecting dna damage

B - Operations – Transporting – 01 – L

Patent

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Details

B01L 3/00 (2006.01) C12Q 1/68 (2006.01) G01N 27/447 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2707662

The invention relates to an apparatus and an in-situ method for detecting cellular DNA damage The invention is particularly suited for use in Comet assays and for automation of such assays The apparatus comprises a multiwell plate, the plate comprising an array of wells held in fixed relationship with each other and each well having an axis, side walls, and a base and wherein the well is provided with electrode pairs disposed therein, and wherein there is provided means for parallel connection of the electrode pairs to an external voltage supply.

La présente invention concerne un appareil et un procédé in situ pour détecter une détérioration d'ADN cellulaire. L'invention est particulièrement appropriée pour être utilisée dans des analyses Comet et pour l'automatisation de telles analyses. L'appareil comprend une plaque à cavités multiples, la plaque comprenant une série de cavités maintenues dans une relation fixe les unes par rapport aux autres, chaque cavité possédant un axe, des parois latérales, et une base, la cavité étant pourvue de paires d'électrodes disposées à l'intérieur, et des moyens étant prévus pour la connexion parallèle des paires d'électrodes à une alimentation en tension externe.

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