Methods and means for nucleic acid sequencing

Details Methods and means for nucleic acid sequencing Methods and means for nucleic acid sequencing

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2559541

Nucleic acid sequencing, especially high-density fingerprinting, in which a panel of nucleic acid probes is annealed to nucleic acid containing a template for which sequence information is desired, with determination of the presence or absence of sequence complementary to each probe within the template, thus providing sequence information. A reference sequence at least partly related to the template is used.

L'invention concerne le séquençage nucléotidique, en particulier la cartographie haute densité, dans lequel un panel de sondes d'acide nucléique est hybridé à de l'acide nucléique contenant une matrice dont on recherche des informations de séquence, avec détermination de la présence ou de l'absence d'une séquence complémentaire à chaque sonde à l'intérieur de la matrice, fournissant ainsi des informations de séquence. Une séquence de référence est utilisée laquelle est au moins partiellement rapportée à la matrice.

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