Cobasorb, a diagnostic test for cobalamin malabsorption

G - Physics – 01 – N

Patent

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G01N 33/82 (2006.01)

Patent

CA 2518896

The present invention relates to a new method named the COBASORB test, which can be used for testing the cause of cobalamin malabsorption in humans. The COBASORB test contains three separate tests (first, second and third test) than can be performed separately, sequentially or in random order and number. The first test use non-radioactive cobalamin for ingestion, the second test uses non-radioactive cobalamin and recombinant intrinsic factor for ingestion and the third test uses recombinant haptocorrin saturated with cobalamin for ingestion. All three tests involve analysis of changes in the concentration of cobalamin saturated transcobalamin (holo-TC) and cobalamin saturated haptocorrin (holo-HC) in the blood. Also disclosed are fits suitable for use in these methods.

L'invention porte sur une nouvelle méthode dite test COBASORB permettant de déceler chez l'homme les causes de la mauvaise absorption de la cobalamine. Ledit test est en trois parties (1, 2 et 3) pouvant se dérouler séparément, séquentiellement ou aléatoirement. La première consiste à ingérer de la cobalamine non-radioactive; la deuxième, à ingérer de la cobalamine non-radioactive et un facteur intrinsèque de recombinaison; et la troisième, à ingérer de l'haptocorrine de recombinaison saturée en cobalamine, les trois parties analysant les variations de concentration dans le sang de la transcobalamine saturée en cobalamine (holo-TC) et de l'haptocorrine saturée en cobalamine (holo-HC). L'invention porte également sur des trousses de mise en oeuvre de la méthode.

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