Lyophilized preparation for synthesis of cell-free protein

C - Chemistry – Metallurgy – 12 – P

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C12P 21/00 (2006.01)

Patent

CA 2485827

Lyophilized preparations from cell extract for use in cell-free protein, which exhibit an activity for protein synthesis being in no way inferior to that exhibited in low-temperature storage; and a method of utilizing the same. In particular, preparations are formed by performing lyophilization after reducing the concentration of deliquescent substances in a solution containing cell extract for synthesis of cell-free protein to a level not detrimental to the quality of preparations after lyophilization.

La présente invention concerne des préparations lyophilisées d'un extrait cellulaire qui sont utilisées dans la synthèse d'une protéine acellulaire, lesquelles préparations présentent une activité de synthèse de protéine qui n'est en aucune manière inférieure à l'activité présente lors d'un stockage à basse température. L'invention se rapporte également à un procédé d'utilisation de ces préparations. En particulier, on forme les préparations de l'invention en procédant à la lyophilisation après avoir réduit la concentration de substances déliquescentes dans une solution contenant un extrait cellulaire destiné à la synthèse d'une protéine acellulaire à un niveau qui ne nuit pas à la qualité des préparations après lyophilisation.

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