A detection method of protein-protein interaction

C - Chemistry – Metallurgy – 12 – N

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C12N 15/79 (2006.01) C07K 19/00 (2006.01) C12P 21/02 (2006.01) G01N 33/50 (2006.01) G01N 33/533 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2374155

A method of detecting an interaction between a protein X and a protein Y having a reporter function in eucaryotic cells, characterized by comprising modifying the protein X and/or the protein Y so that the proteins X and Y are expressed in cells in different localization patterns, co-expressing the proteins X and Y in eucaryotic cells, and then specifying the intracellar localization of the protein Y by using the reporter function as an indication; and a method of detecting the inhibiting or promoting effect of a compound Z on the interaction of the proteins X and Y in eucaryotic cells.

Un procédé de détection d'une interaction entre une protéine X et une protéine Y ayant une fonction reporteur dans des cellules eucaryotes se caractérise en ce qu'on modifie la protéine X et/ou la protéine Y de sorte que les protéines X et Y soient exprimées dans des cellules suivant des types de localisation différents, en ce qu'on co-exprime les protéines X et Y dans des cellules eucaryotes et en ce qu'on spécifie la localisation intracellulaire de la protéine Y en utilisant la fonction reporteur en tant qu'indication. On décrit également un procédé de détection de l'effet inhibiteur ou promoteur d'un composé Z sur l'interaction des protéines X et Y dans des cellules eucaryotes.

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