Novel method for gene cloning

C - Chemistry – Metallurgy – 40 – B

Patent

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C40B 40/08 (2006.01) C07K 14/705 (2006.01) C07K 16/00 (2006.01) C07K 16/28 (2006.01) C07K 19/00 (2006.01) C12N 15/10 (2006.01) C12N 15/12 (2006.01) C12N 15/13 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2331917

A method for isolating a gene encoding a membrane-bound protein characterized by fusing a functional protein with a fused protein per se, differing from the existing TMT method wherein an epitope recognized by an antibody is carried in a fused protein. This method makes it possible to selectively isolate a gene encoding a membrane-bound protein.

L'invention concerne un procédé permettant d'isoler un gène codant une protéine délimitée par une membrane. Ce procédé consiste à assurer la fusion d'une protéine fonctionnelle avec une protéine ayant déjà subi une fusion. Ce procédé se différencie du procédé TMT actuel, selon lequel un épitope reconnu par un anticorps est supporté dans une protéine ayant subi une fusion. Ce procédé permet d'isoler, de manière sélective, un gène codant une protéine délimitée par une membrane.

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