C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2515040
The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.
La présente invention concerne des méthodes destinées à prétraiter des échantillons biologiques afin d'en extraire un acide nucléique. La présente invention fait appel à une combinaison d'au moins un agent de dénaturation protéique avec l'un ou plusieurs des éléments suivants en vue de la formation d'un mélange de réaction destiné à l'extraction d'un acide nucléique : (1) au moins un solvant aprotique, (2) un chauffage progressif, et (3) une dilution d'échantillon.
Collis Matthew
Copertino Donald
Eckert Karen
Fort Thomas
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
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