Polyubiquitin based hydrogel and uses thereof

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/00 (2006.01) A61K 47/42 (2006.01) A61L 15/32 (2006.01)

Patent

CA 2409188

The present invention relates to a novel biopolymer consisting of a three- dimensional cross-linked mixture of (a) a cross-linking agent, activated with an activating agent, dissolved in a aqueous solution, and (b) a recombinant protein, namely polyubiquitin. The novel biopolymer is based on the cross- linking of ubiquitin (monomeric and/or polymeric) with a cross-linking agent, preferably bifunctionalized polyethylene oxides or a polyethylene glycol of various molecular masses (MW 2000 to 35 000 kDa), dissolved in aqueous solution in adequate proportions. The novel biopolymer offers a wide range of formulations since the number of ubiquitin units and cross-linking agent can vary both in length and ratio. The novel hydrogel is also biodegradable by a specific protease and is resistant to a wide range of other proteases.

La présente invention concerne un nouveau biopolymère constitué d'un mélange réticulé en trois dimensions d'un (a) agent de réticulation activé avec activateur, dissout dans une solution aqueuse et, (b) d'une protéine de recombinaison, à savoir la polyubiquitine. Ce nouveau biopolymère est fondé sur la réticulation de l'ubiquitine (monomère et/ou polymère) avec un agent de réticulation, de préférence des oxydes polyéthylènes bifonctionnalisés ou un polyéthylène glycol de masses moléculaires diverses (MW 2000 à 35,000 kDa), dissous dans une solution aqueuse en proportions adéquates. Ce nouveau biopolymère offre une grande gamme de préparations car le nombre d'unités d'ubiquitine et d'agent de réticulation peut varier en quantité et en rapport. Ce nouvel hydrogel est également biodégradable par une protéase spécifique et il est résistant à une grande gamme d'autres protéase.

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