Promoter for introducing extracellular substance into...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/09 (2006.01) A01K 67/02 (2006.01) A61K 45/00 (2006.01) A61P 15/00 (2006.01) A61P 15/08 (2006.01)

Patent

CA 2607772

It is intended to provide a method, upon introducing an extracellular substance such as a sperm, a cell nucleus, a nucleic acid or a protein into a mammalian ovum, for introducing the extracellular substance into the mammalian ovum, which is safe for the mammalian ovum, enables efficient introduction, and is technically easy. By treating the mammalian ovum with a tubulin polymerization inhibitor such as vinblastine, membrane fusion between the ovum and the sperm is promoted and without making a hole in the cell membrane, the sperm and the ovum are fused and fertilization can be achieved. The method of the invention can be applied not only to the case where a sperm is introduced into a mammalian ovum, but also to a case where an extracellular substance such as a cell nucleus, a nucleic acid or a protein is introduced into a mammalian ovum.

L'invention a pour but de proposer un procédé, lors de l'introduction d'une substance extracellulaire telle que du sperme, un noyau cellulaire, un acide nucléique ou une protéine dans un ovule de mammifère, destiné à introduire la substance extracellulaire dans un ovule de mammifère, qui est sans danger pour l'ovule de mammifère, permet une introduction efficace et est facile d'un point de vue technique. Le traitement de l'ovule de mammifère avec un inhibiteur de la polymérisation de tubuline, tel qu'une vinblastine, favorise une fusion de membrane entre l'ovule et le sperme et sans trouer la membrane cellulaire, le sperme et l'ovule sont fusionnés et la fertilisation peut être obtenue. Le procédé de l'invention peut être appliqué non seulement au cas où du sperme est introduit dans un ovule de mammifère, mais aussi à un cas où une substance extracellulaire, telle qu'un noyau cellulaire, un acide nucléique ou une protéine est introduit(e) dans un ovule de mammifère.

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