Culturing apparatus and cultivating method

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/04 (2006.01) C12M 1/16 (2006.01) C12M 1/38 (2006.01)

Patent

CA 2294901

Disclosed is a culturing apparatus (100) that includes a gas-permeable solid phase culturing bed (110) disposed within an interior space of a vessel (108). The culturing bed comprises a solid substrate that includes a biological agent disposed thereon. A gas inlet (115, 116) permits selective introduction of gas into the vessel (108) under pressure sufficient to cause gas to flow through at least a portion of the bed (110). Also disclosed is a method of cultivating a biological agent. The method comprises providing a gas-permeable culturing bed, flowing a gas through the bed and recovering at least a portion of the biological agent. The gas preferably is an oxygen-containing gas, whereby the flow of gas through the bed provides convective mass transfer of oxygen to the biological agent. The apparatus and method of the invention permit "pressure pulsing" of gas through the bed.

L'invention concerne un appareil (100) de culture comprenant un lit (110) de culture en phase solide perméable aux gaz disposé dans l'espace intérieur d'un récipient (108). Le lit de culture comprend un substrat solide sur lequel est disposé un agent biologique. Un orifice (115, 116) d'entrée de gaz permet l'introduction sélective de gaz dans le récipient (108) à une pression suffisante pour provoquer l'écoulement du gaz sur une partie du lit (110) au moins. L'invention concerne également un procédé de culture d'un agent biologique. Ledit procédé consiste à prendre un lit de culture perméable aux gaz, à faire passer un gaz à travers ce lit et à récupérer au moins une partie de l'agent biologique. Le gaz est de préférence un gaz renfermant de l'oxygène, ce qui explique que l'écoulement de gaz à travers le lit entraîne un transfert de masse par convection d'oxygène vers l'agent biologique. L'appareil et le procédé de l'invention permettent d'obtenir une "pression pulsée" du gaz à travers le lit.

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