A method to map and isolate regions of chromosomes that...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2292728

The present invention relates to a method to identify regions of chromosomes that interact functionally within or between chromosomes which comprises the steps of: a) recombining a linear recipient vector with a target sequence present in at least one chromosome, wherein the recipient vector comprises at least a recipient sequence consisting of a nucleic acid sequence complementary to recombine with the target sequence and a tail consisting of a heterologous nucleic acid sequence; and b) identifying at least one region of the chromosome capable of recombining with the recipient vector by PCR means or by detecting a label and/or a tag on the recipient vector or the target sequence.

L'invention concerne une méthode permettant d'identifier des régions chromosomiques qui interagissent fonctionnellement à l'intérieur d'un chromosome ou entre plusieurs chromosomes. Selon la méthode: a) on recombine un vecteur receveur linéaire avec une séquence cible présente dans au moins un chromosome, le vecteur receveur renfermant au moins une séquence receveuse constituée d'une séquence d'acide nucléique complémentaire, capable de se recombiner avec la séquence cible, et une queue constituée d'une séquence d'acide nucléique hétérologue; et b) on identifie au moins une région chromosomique capable de se recombiner avec le vecteur receveur en utilisant la PCR ou en détectant un marqueur et/ou une étiquette sur ledit vecteur ou ladite séquence cible.

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