Method of fractionating plasma protein

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

530/15.06, 530/3

C07K 1/36 (2006.01) C07K 1/22 (2006.01)

Patent

CA 2019893

ABSTRACT OF THE DISCLOSURE A method of fractionating plasma protein which comprises treating a plasma protein-containing material in the following sequence of steps, provided that steps (ii) through (v) may be performed in an optional order: (i) Freeze-thaw treatment (ii) Treatment at 5 to 10% ethanol concentration (iii) Treatment with an anion exchanger (iv) Affinity chromatography with immobilized lysine (v) Affinity chromatography with immobilized heparin (vi) Treatment at 18 to 30% ethanol concentration (vii) Treatment at 35 to 45% ethanol concentration

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