Leptin assay

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2386365

The invention provides a method for determining the presence of leptin, based on detecting leptin-induced angiopoietin-2 expression in some cells or cell lines, e.g. murine Swiss 3T3 F442A preadipocytes or human marrow stromal cell line hMS2-12. Leptin detection is e.g. performed transfecting cells with a vector comprising a reporter gene (e.g. Green Fluorescent Protein (GFP)) under the control of angiopoietin-2 promoter.

La présente invention concerne une méthode permettant de déterminer la présence de leptine dans un échantillon.

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