Multi-immunoaffinity based antigen identification

Details Multi-immunoaffinity based antigen identification Multi-immunoaffinity based antigen identification

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2686418

The present invention relates to methods for identifying antigens from complex analyte samples. More specifically, the invention relates to methods for identifying antigens recognized by monoclonal antibodies in a complex analyte sample. The methods of this invention use a first affinity chromatography via a "multiaffinity" step and a second, "singleaffinity" step that implements parallel affinity chromatography.

La présente invention concerne des méthodes permettant d'identifier des antigènes d'échantillons d'analytes complexes. Plus particulièrement, l'invention concerne des méthodes permettant d'identifier des antigènes reconnus par des anticorps monoclonaux dans un échantillon d'analyte complexe. Les méthodes de cette invention utilisent une première chromatographie d'affinité par une étape d'affinité multiple et une seconde étape d'affinité simple qui met en AEuvre une chromatographie d'affinité en parallèle.

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