Genes encoding denitrification enzymes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 9/06 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2401484

This invention relates to the isolation of nucleic acid fragments from Methylamons sp. that encode enzymes involved in denitrification. The enzymes are useful in denitrification reactions and for the identification of other denitrifying bacteria. In addition, this invention also relates to the construction of chimeric genes encoding all or a substantial portion of the present genes in sense or antisense orientation, wherein the expression of the chimeric genes results in production of altered levels of the present gene products in the recombinant host.

L'invention concerne l'isolement de fragments d'acide nucléique de Methylamons sp. qui codent les enzymes impliquées dans la dénitrification. Lesdites enzymes sont utiles dans les réactions de dénitrification et pour l'identification d'autres bactéries de dénitrification. Par ailleurs, l'invention porte sur la construction de gènes chimères codant pour tous les gènes présents ou pour une partie de ceux-ci selon une orientation sens ou antisens, l'expression des gènes chimères induisant la production de niveaux modifiés des produits géniques présents de l'hôte recombiné.

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