Methods and apparatuses for convective polymerase chain...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C12M 1/34 (2006.01) C12M 1/38 (2006.01)

Patent

CA 2713021

The present invention provides a method and apparatus for amplifying a nucleic acid sequence by polymerase chain reaction (PCR). The method comprises placing a PCR sample in a container which is heated by only a single heat source that provides a high temperature for denaturation in the bottom of the PCR sample, while annealing and extension automatically occur in different regions of the PCR sample due to the convection induced by a temperature gradient descending from the bottom of the PCR sample to the surface of the PCR sample.

La présente invention concerne un procédé et un appareil pour amplifier une séquence d'acide nucléique par une réaction en chaîne par polymérase (PCR). Le procédé comprend la mise en place d'un échantillon de PCR dans un récipient qui est chauffé seulement par une source de chaleur unique qui fournit une température élevée en vue de la dénaturation dans le fond de l'échantillon de PCR, alors que le recuit et l'extension surviennent automatiquement dans différentes zones de l'échantillon de PCR du fait de la convection induite par un gradient de température descendant du fond de l'échantillon de PCR à la surface de l'échantillon de PCR.

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